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                                                            Braun Lab

                                                            Max Eder Junior Research Group

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                                                            Research topics

                                                            • Targeted therapy for childhood cancers
                                                            • Genome-wide genetic screens
                                                            • Role of RNA life cycle in cancer biology
                                                            • Personalized therapy of pediatric tumors


                                                            Dr. med. Christian J. Braun

                                                            ✉ chr.braun@med.lmu.de

                                                            ☎ +49-89-4400-54724

                                                            We are trying to understand what mechanisms are employed by childhood cancers to fuel their oncogenic signaling machinery. In order to do so, we employ state of the art model systems for pediatric tumors, high-throughput CRISPR and RNAi screens, comprehensive analyses of transcriptional landscapes as well as customized experimental pipelines for the establishment of biomarker profiles.

                                                            Our group is particularly interested in discovering and characterizing new targetable vulnerabilities to advance the therapy of pediatric nervous system tumors such as glioma and neuroblastoma.

                                                            RNA life cycle
                                                            Authorship unknown

                                                            All classes of cellular RNAs are subject to direct and post-transcriptional modification. RNA binding proteins (RBPs) specifically recognize many of these modifications and consecutively influence RNA stability, alternative splicing, location and decay. There is emerging evidence that both developing tissues and cancer cells utilize these ‘epitranscriptional’ processes to orchestrate gene expression profiles in a coordinated fashion (Braun and Hemann, Curr Opin Genet Dev, 2019). 

                                                            Not surprisingly, core ‘epitranscriptional’ regulators such as splice factors are frequently subject to mutation in cancer. We have previously found that the splicing rate of specific introns contributes to gene expression orchestration in both physiology and cancer. Intriguingly, this process represents a targetable vulnerability (Braun et al., Cancer Cell, 2017).

                                                            Our lab is interested in deeper characterizing this observation and in systematically analyzing the role of post-transcriptional RNA biology in childhood cancer.

                                                            Functional screens
                                                            Authorship unknown

                                                            We are trying to understand what mechanisms are employed by childhood cancers to fuel their oncogenic signaling machinery. In order to do so, we employ state of the art model systems for pediatric tumors, high-throughput CRISPR and RNAi screens, comprehensive analyses of transcriptional landscapes as well as customized experimental pipelines for the establishment of biomarker profiles. We have successfully applied these experimental systems to several disease entities such as brain cancer, lymphoma and leukemia in the past.

                                                            Our group is particularly interested in discovering and characterizing new targetable vulnerabilities to advance the therapy of pediatric nervous system tumors such as glioma and neuroblastoma, but are also eager to collaborate on applying our screening technologies to biological questions far away from molecular oncology. 

                                                            Therapy biomarkers

                                                            Many targeted cancer therapies fail in clinical trials – which is dilemma for patients, society and health care economy. In general, the effectiveness of targeted therapies tends to fall behind expectations when there is no stratifying biomarker available enabling clinicians to restrict treatment to sensitive sub-cohorts of patients. When characterizing novel therapeutic vulnerabilities, we therefore aim to systematically establish gene expression signatures predictive of therapeutic sensitivity


                                                            Dr. med. Christian Braun

                                                            Group Leader

                                                            ✉ chr.braun@med.lmu.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.05


                                                            Andres Carbonell Adames, M. Sc.

                                                            Doctoral Researcher (PhD track)

                                                            M.Sc. LMU Munich, B. Sc. Penn State, U.S..A

                                                            ✉ Andres.Carbonell@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31


                                                            Annika Danker, Cand. med.

                                                            Doctoral Researcher (MD track)

                                                            ✉ Annika.Danker@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31


                                                            Daniel Schäfer, Cand. med.

                                                            Doctoral Researcher (MD track)

                                                            Jointly supervised with Kimberly Stegmaier
                                                            at Dana-Farber Cancer Institute / Harvard Medical School




                                                            Heidi Noll-Puchta, VTA

                                                            Technician & Lab Manager

                                                            ✉ Heidi.Noll-Puchta@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31


                                                            Mark Rassner,
                                                            Dr. med. Dipl.-Mol. med.

                                                            Clinician Scientist 

                                                            ✉ Mark.Rassner@med.lmu.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31


                                                            Liana Gahleitner, Cand. med.

                                                            Doctoral Researcher (MD track)

                                                            ✉ Liana.Gahleitner@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31


                                                            Dorothea Mock, Cand. med.

                                                            Doctoral Researcher (MD track)

                                                            ✉ dorothea.mock@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31

                                                            Alumni


                                                            John Ku, B. Sc. 

                                                            Master Thesis Student

                                                            B. Sc. California State Polytechnic University, USA 

                                                            ✉ John.Ku@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31


                                                            Satyendra Kumar, PhD

                                                            Postdoctoral Researcher

                                                            PhD Karolinska Institute, Sweden

                                                            ✉ satyendra.kumar@med.uni-muenchen.de

                                                            ☎ 089-4400-54724

                                                            Room: K0.31

                                                            Selected Publications

                                                            C. J. Braun*, A. C. Adames, D. Saur & R. Rad*. Tutorial: design and execution of CRISPR in vivo screens. *Co-corresponding

                                                            Nature protocols (2022)
                                                            Journal Impact Factor2021: 17.021     

                                                                        >> Selected "Paper of the month" by LMU's medical faculty    Link

                                                            DOI

                                                            T. Kaltenbacher, J. Loprich, R. Maresch, J. Weber, S. Muller, R. Oellinger, N. Gross, J. Griger, N. de Andrade Kratzig, P. Avramopoulos, D. Ramanujam, S. Brummer, S. A. Widholz, S. Barthel, C. Falcomata, A. Pfaus, A. Alnatsha, J. Mayerle, M. Schmidt-Supprian, M. Reichert, G. Schneider, U. Ehmer, C. J. Braun, D. Saur, S. Engelhardt, and R. Rad. CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver.

                                                            Nature protocols (2022)
                                                            Journal Impact Factor2021: 17.021

                                                            DOI

                                                            J. Weber, C. J. Braun, D. Saur, R. Rad. In vivo functional screening for systems-level integrative cancer genomics. In vivo functional screening for systems-level integrative cancer genomics.

                                                            Nature Reviews Cancer (2020)
                                                            Journal Impact Factor2021: 69.800

                                                            DOI

                                                            M. Becker, H. Noll-Puchta, D. Amend, F. Nolte, C. Fuchs, I. Jeremias*, C. J. Braun*. CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries.

                                                            Nucleic Acids Research (2020)
                                                            Journal Impact Factor2021: 19.160

                                                            DOI

                                                            C. J. Braun*, M. Stanciu*, P. L. Boutz*, J. C. Patterson, D. Calligaris, F. Higuchi, R. Neupane, S. Fenoglio, D. P. Cahill, H. Wakimoto, N. Y. R. Agar, M. B. Yaffe, P. A. Sharp, M. T. Hemann, J. A. Lees. Coordinated Splicing of Regulatory Detained Introns within Oncogenic Transcripts Creates an Exploitable Vulnerability in Malignant Glioma. *equal contributions

                                                            Cancer Cell (2017)

                                                            Journal Impact Factor2021: 38.585

                                                            DOI

                                                            C. J. Braun*, P. M. Bruno*, M. A. Horlbeck, L. A. Gilbert, J. S. Weissman, M. T. Hemann. Versatile in vivo regulation of tumor phenotypes by dCas9 mediated transcriptional perturbation. *equal contributions

                                                            PNAS (2016)

                                                            Journal Impact Factor2021: 10.700

                                                            DOI

                                                            I. G. Cannell, K. A. Merrick*, S. Morandell*, C. Z. Zhu, C. J. Braun, R. A. Grant, E. R. Cameron, M. Tsao, M.T. Hemann and M. B. Yaffe. A Pleiotropic RNA-Binding Protein Controls Distinct Cell Cycle Checkpoints to Drive Resistance of p53-defective Tumors to Chemotherapy.

                                                            Cancer Cell (2015)

                                                            Journal Impact Factor2021: 38.585

                                                            DOI

                                                            C. J. Braun*, K. Boztug*, A. Paruzynski*, M. Witzel*, A. Schwarzer, M. Rothe, U. Modlich, R. Beier, G. Göhring, D. Steinemann, R. Fronza, C. R. Ball, R. Haemmerle, S. Naundorf, K. Kühlcke, M. Rose, C. Fraser, L. Mathias, R. Ferrari, M. R. Abboud, W. Al-Herz, I. Kondratenko, L. Maródi, H. Glimm, B. Schlegelberger, A. Schambach, M. H. Albert, M. Schmidt, C. von Kalle, and C. Klein. Gene Therapy for Wiskott-Aldrich Syndrome--long-term efficacy and genotoxicity. *equal contributions

                                                            Science translational medicine (2014)

                                                            Journal Impact Factor2021: 19.359

                                                            DOI

                                                            C. P. Pallasch, I. Leskov, C. J. Braun, D. Vorholt, A. Drake, Y. M. Soto-Feliciano, E. H. Bent, J. Schwamb, B. Iliopoulou, N. Kutsch, N. van Rooijen, L. P. Frenzel, C. M. Wendtner, L. Heukamp, K. A. Kreuzer, M. Hallek, J. Chen, and M. T. Hemann. Sensitizing Protective Tumor Microenvironments to Antibody-Mediated Therapy.

                                                            Cell (2014)

                                                            Journal Impact Factor2021: 66.850

                                                            DOI
                                                            All publications Braun Lab

                                                            CLUE: Multiplexed sgRNA library design and production

                                                            Pooled sgRNA libraries targeting hundreds or thousands of individual genes are right at the heart of functional genomics. Such libraries are broadly available, but usually not tailored to the scientific interest of researchers. With our tool CRISPR clue were were out to bring custom sgRNA library production to all labs - streamlining the cloning process, lowering synthesis costs and reducing bioinformatics to the bare minimum. Check out www.crispr-clue.de and the manuscript at Nucleic Acids Research (NAR)! 

                                                            Andres' podcasts

                                                            Andres is very passionate about making science & molecular biology accessible to the general public. Check out his podcasts!

                                                            Youtube
                                                            Spotify

                                                            Christian's tweets

                                                            Join us!

                                                            We are highly enthusiastic about science - but also love to teach, discuss, collaborate and hang out.

                                                            If you are interested in CRISPR screens, functional genomics, genetic crosstalk, context dependence, translation into clinic and the discovery of new treatment opportunities for childhood cancers: please don't hesitate to send an email to Christian (chr.braun@med.lmu.de) or any of the current lab members. 

                                                            Christian Braun Lab


                                                            Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital,

                                                            Klinikum der Universität München, LMU München


                                                            Postal Address: Lindwurmstr. 4

                                                            Visiting Address: Research Building, Lindwurmstraße 2a

                                                            80337 Munich

                                                            Germany


                                                            Room: K 0.5

                                                            Phone: +49 (0)89 - 4400 - 54724

                                                            Fax: +49 (0)89 - 4400 - 57702

                                                            Email: chr.braun@med.lmu.de


                                                            Lab Manager: Heidi Noll-Puchta

                                                            Postal Address: Lindwurmstr. 4

                                                            Visiting Address: Research Building, Lindwurmstraße 2a

                                                            80337 Munich

                                                            Germany


                                                            Room: K 0.31

                                                            Phone: +49 (0)89 - 4400 - 54724

                                                            Fax: +49 (0)89 - 4400 - 57702

                                                            Email: Heidi.Noll-Puchta@med.uni-muenchen.de

                                                            Research at CCRC Hauner

                                                            Contact LMU Klinikum

                                                            Contact CCRC Hauner

                                                            Haunersches

                                                            CCRC Hauner

                                                            Kinderklinik und Kinderpoliklinik

                                                            im Dr. von Haunerschen Kinderspital

                                                            Ludwig Maximilians Universität München

                                                            Lindwurmstr. 4

                                                            80337 Munich, Germany

                                                            ☎ +49-89-4400-57705

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                                                              • Scivias Study
                                                              • TRACE Study
                                                            • PhD Program
                                                            • Technology platforms
                                                              • Bioinformatics
                                                              • Flow Cytometry
                                                              • High throughput sequencing
                                                              • Microscopy
                                                              • Pre-GMP Facility
                                                              • Mass spectrometry
                                                              • Hauner Biobank
                                                            • News/Events
                                                              • Meinhard von Pfaundler-Lectures
                                                              • Klaus Betke Symposium
                                                            • Join us!